7 December 2021

Investigation of the effect of different sensitization agents used in the treatment of dentinal sensitivity in closure of dentinal tubules: Scanning electron microscopy analysis

Tag

Yenituran, Ahmet. (2022). Investigation of the effect of different sensitization agents used in the treatment of dentinal sensitivity in closure of dentinal tubules: scanning electron microscopy analysis. (Unpublished master's thesis). Altınbaş University, Graduate School of Education, Istanbul.

Summary

Objective: In this study, it was aimed to investigate the in-vitro effects of toothpastes containing sodium fluoride (NaF), %8 arginine and calcium carbonate, nanohydroxyapatite (n-Hap) on the closure of dentinal tubules using scanning electron microscopy (TEM). Materials and Methods: 20 dentin specimen sections obtained from impacted third molars extracted into the study were divided into 4 different groups, each consisting of 5 specimens. In order to remove the smear layer, %1 citric acid was first applied to the surface of all dentin samples and the dentin tubule entrances were exposed. Afterwards, no treatment was applied to the dentin samples in group I to create a control group. II. Toothpaste containing NaF, III. Sensitivity toothpaste containing %8 arginine and calcium carbonate and IV. Toothpaste containing n-HAp was applied to the samples in the group. All samples were examined by TEM for occlusion of dentinal tubules and tubule morphology. Results and Conclusion: The mean amount of dentinal tubules, the diameter of dentinal tubules and the amount of tubules per 100 µm2 exposed in the microphotographs at x2000 magnification taken from the samples were found to be 375.2, 2.55 µm and 1.2 in the control group, respectively. These values were found to be 3.6, 0.13 µm, 0.01 , respectively in group II, 0.4, 0.04 µm, and 0.001 in group III, and 29.4, 0.51 µm, 0.09 in group IV, respectively.

In the comparison between the groups, the maximum values obtained as the amount of exposed dentinal tubules, the diameter of the dentinal tubules and the amount of tubules per 100 µm2 were found in group I, group IV, group II and group III, respectively. The results obtained in this study showed that all treatment options applied were effective in closing the dentinal tubules, changing the dentin surface morphologies and narrowing the dentinal tubule diameters. .